Since live and dead cells can be discriminated using a single dye and a single channel of a flow cytometer, it is an ideal choice for multi-color experiments. The near-IR fluorescent reactive dye has an excitation maximum of ∼633 nm making it ideal for use with the red or HeNe laser with an emission of ∼750 nm. The LIVE/DEAD™ Fixable Near-IR stain was selected based on its fluorescent properties to provide a bright signal when excited with a red laser. The LIVE/DEAD™ Fixable Near-IR Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines, and the staining pattern is preserved following formaldehyde fixation. If this is not possible, aliquot the vials in small volumes and store at -80☌, avoiding freeze-thaw cycles.ĭead cell discriminator stains can lose sensitivity after treatment with fixatives such as formaldehyde or ethanol-based fixation methods required for intracellular phosphorylation studies. Amine reactive dyes in solution will lose their effectiveness over a short period of time, therefore it is recommended to completely use the vial once rehydrated.
Unlike products that are sold in solution, the LIVE/DEAD™ Fixable Near-IR Stain has been conveniently packaged in 40-test vials to help ensure the stability and performance of the dye over time. View a selection guide for all fixable viability dyes for flow cytometry.
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